Large-scale preparation of endotoxin-free plasmid DNA using NucleoBond Xtra Midi EF Kit (Macherey Nagel 740420.10) was carried out. We then used the ExpiCHO Expression System (Thermo Fisher Scientific A21933) to produce soluble nanobody-Fc fusion proteins. The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt.
Expression Medium: CHOgro ® Expression Medium is a chemically defined, serum-free growth medium that permits high density growth and large-scale transfection of suspension CHO cells. Many suspension CHO cells (e.g. FreeStyle™ CHO-S and ExpiCHO-S™) readily adapt to supplemented CHOgro ® Expression Medium through sequential adaptation.