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Expression Medium: CHOgro ® Expression Medium is a chemically defined, serum-free growth medium that permits high density growth and large-scale transfection of suspension CHO cells. Many suspension CHO cells (e.g. FreeStyle™ CHO-S and ExpiCHO-S™) readily adapt to supplemented CHOgro ® Expression Medium through sequential adaptation.

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Nov 13, 2007 · The calcium content of the growth medium has been shown to influence the growth and differentiation of primary epithelial cells in culture. The goal of the present study was to determine if growth medium calcium concentration could influence the susceptibility to metal toxicity and metallothionein gene expression of an immortalized human prostate-derived epithelial cell line (RWPE-1).

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Large-scale preparation of endotoxin-free plasmid DNA using NucleoBond Xtra Midi EF Kit (Macherey Nagel 740420.10) was carried out. We then used the ExpiCHO Expression System (Thermo Fisher Scientific A21933) to produce soluble nanobody-Fc fusion proteins. The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt.

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CHO Expression Medium. This medium should be supplemented with L-glutamine to a final concentration of 8 mM before use (e.g., 40 mL of 200 mM stock to one liter of medium). • 5 mL/L of Penicillin/Streptomycin (0.5X Pen Strep) may be used when required

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ExpiCHO Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal originfree medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO Expression System Kit

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